Inactivation of protein tyrosine phosphatases by dietary isothiocyanates.
نویسندگان
چکیده
Isothiocyanates are bioactive dietary phytochemicals that react readily with protein thiol groups. We find that isothiocyanates are time-dependent inactivators of cysteine-dependent protein tyrosine phosphatases (PTPs). Rate constants for the inactivation of PTP1B and SHP-2 by allyl isothiocyanate and sulforaphane range from 2 to 16 M(-1)s(-1). Results in the context of PTP1B are consistent with a mechanism involving covalent, yet reversible, modification of the enzyme's active site cysteine residue.
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ورودعنوان ژورنال:
- Bioorganic & medicinal chemistry letters
دوره 25 20 شماره
صفحات -
تاریخ انتشار 2015